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SF3B1 pull down assay coupled to mass spectrometry analysis 1 g of purified GST-tagged SF3B1(1463) in 48 l of kinase buffer (20 mM HEPES-KOH pH=7.9, 5 mM MgCl 2 , 2 mM DTT, 2 mM ATP, 1x phosphatase inhibitor cocktail (Sigma, P0044)) was incubated with 12 l flag-tagged CDK11 kinase (immunoprecipitated from HEK293 cells) or with 12 l of elution buffer (20 mM HEPES-KOH pH=7.9, 150 mM KCl, 1 mM DTT)

Selective cytotoxic effect may be attributed due to the pH sensitivity and oxidative stress generation due to genes modulation, which is involved in ROS generation metabolism, apoptosis and mitochondrial dysfunction [233]

To maximize safety, use qualified providers and stick to recommended doses

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[DOI] [PubMed] [Google Scholar] 218.Zhang T, Yang D, Fan Y, Xie P, Li H